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Copenhagen,
Denmark
19 - 22 June 2005
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Microarray
and real-time PCR analysis: comparison between
ovarian endometriosis and ovarian cancer
C
Banz [1], H Xu [1], U Ungethuem [2], RJ Kuban
[3],
F Köster [1], R Felberbaum [1], K Diedrich
[1], D Hornung [1]
[1] Universität Lübeck
Frauenklinik
Lübeck, Germany
[2]
Charite, Labor für funktionelle Genomforschung
Berlin, Germany
[3] Charite, Biochemisches Institut
Berlin, Germany
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Introduction
Women with endometriosis have an increased risk
to develop endometrioid or clear-cell ovarian cancer.
If endometriosis patients suffer additionally from
primary infertility, the incidence rate is increased
more than four times. To identify possible differences
in gene expression between ovarian endometriosis,
ovarian cancer and benign ovaries, we used total
RNA in microarray technology.
Materials and methods
Total RNA was extracted from 5 samples each of ovarian
endometriosis, ovarian cancer and normal ovaries,
cRNA samples hybridized to the Affymetrix HG-U133A
microarray chip and mRNA levels of over 22,000 genes
assessed. Random genes were validated by real time
PCR, including additional samples to enlarge the
number of patients.
Results
Eight hundred and twenty-three genes were statistically
differentially expressed in all three tissues types,
with the following biological groups being differentially
expressed with the highest percentage per group:
regulation of G-protein coupled receptor protein
signaling pathway, angiogenesis, cell cycle arrest,
MAPKKK cascade,protein kinase cascade and cytokinesis.
Genes like Angiopoietin 1,small inducible cytokine
A2 (SICA2) and small inducible cytokine subfamily
A member 14 (CCL14) showed significant differences
within the three examined groups.
Conclusions
Our study described several genes differentially
expressed in ovarian endometriosis, ovarian cancer
and healthy ovaries. Other genes that are equally
expressed in ovarian endometriosis and ovarian cancer,
might be involved in the development and progression
of these diseases. Further studies are necessary
to show if it is possible to identify endometriosis
patients with a higher risk for ovarian cancer later
in life, and offer prophylactic treatment. Therefore,
we are planning to investigate gene expression in
ovarian cancer of former endometriosis patients.
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